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The aim of the following research was to analyse the presence of mutation in gyrA gene in C. jejuni and C. coli strains isolated from broilers, laving hens, turkeys collected between 1994-1996 and 2005-2008, and isolated from humans in 2006. The results of the research demonstrated that the highest percentage (100%) of the isolates showing mutation in gyrA gene was observed in Campylobacter coli strains...
Sixty-one isolates of M. bovis (58 from cattle and three from wild animals) from eight regions of Poland were analysed. Molecular analysis was done using HAIN and spoligotyping methods. Drug susceptibility of the isolates to streptomycin, izoniazid, rifampicin, ethambutol, and pyrazinamide was tested by proportional methods on solid and liquid media. By spoligotyping, 47 (77%) isolates were identified...
The hypothesis that acute pancreatitis in dogs could be diagnosed based on the ratio of urinary α-amylase activity to creatinine concentration (U-A/C) was tested. The study was performed on 292 dogs. Based on clinical, laboratory , and imaging findings, the dogs were divided into the following groups: 34 healthy patients serving as the control, 48 sick dogs with U-A/C>2.9, and 210 sick dogs with...
Eighteen purebred Arabian mares and six Anglo-Arabian mares were observed during first 4 h after foaling in one of the leading Polish stud farms in two foaling seasons. The aim of the research was to analyse the behaviour of mares and the condition of foals after parturition. Specific behavioural responses like licking, nuzzling, and touching a foal were identified as interactive activities supporting...
A liquid chromatographic method coupled with UV-Vis and fluorescence detectors for the determination of azaperone, carazolol, and chlorpromazine residues in urine has been described. Analytes were isolated from biological samples with acetonitrvle. The obtained extracts were applied to solid phase extraction Strata X-C 33 µn Polimeric Strong Cation columns. The analytes were eluted with alkalised...
The influence of L-arginine and Nω-nitro-L-arginine (L-NNA) on lead-induced oxidative stress in kidneys of rats with different resistance to hypoxia was studied. The animals were exposed to lead nitrate (3.6 mg/kg, daily) for 30 d and treated with L- arginine (600 mg/kg) or L-NNA (35 mg/kg) 30 min before each injection of lead nitrate. Lead exposure led to a significant increase in the level of thiobarbituric...
The following fish samples were examined: control salted samples (A), salted samples with 0.1% of thyme oil (B), (salted samples with 0.3% of thyme oil (C), and salted samples with 0.5% of thyme oil (D).The study was based on microbiological (total viable count (TVC), Pseudomonas sp., lactic acid bacteria (LAB), Enterobacteriaceae, and H₂S-producing bacteria), chemical (total volatile basic nitrogen...
The influence of anaemia on alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) activities in dogs infected with B. canis was investigated. Samples of blood and serum from 230 infected dogs were divided into two groups: A (with anaemia) and B (without anaemia). The differences in the activities of the enzymes between both groups were not statistically significant...
The aim of this study was the assessment of the usefulness of the ultrasound-guided method during injections into the Th₄- Th₇ intercostal nerve area in dogs, as well as comparison of the liquid deposition precision obtained using anatomical and ultrasound- guided methods. The study has been performed on fresh cadavers of 20 dogs. A mixture of black ink and iodinated contrast medium was injected....
The response of five positive acute phase proteins (APP) was evaluated in pigs after infection with Bordetella bronchiseptica (Bbr). Twelve piglets from a herd with high health status were used. Six of them were infected intranasally with Bbr (3.4 x 10⁸ cfu). The standard bacteriological methods and PCR were used for detection of Bbr in nasal swabs, lungs, and bronchoalveolar fluids. Serum APP concentrations...
Two hundred and forty-five samples of honey representing different botanical types were microbiologically tested for total number of aerobic bacteria, Salmonella sp., anaerobic bacteria, yeasts, and moulds. The samples presented different level of microbiological contamination. Total number of aerobic bacteria varied from 1.0 x 10¹ cfu/g to 7.5 x 10⁴ cfu/g. Salmonella sp. was not detected in any of...
The main aim of this study was to evaluate the prevalence of campylobacteriosis in population of Polish heifers. An additional objective was to compare two independent methods used in diagnosis of the disease: culture and PCR. The 1,600 vaginal swabs from heifer origin from different farms of Poland were investigated using both methods. The results showed that the percentage of positive samples was...
Investigation was carried out on red poultry mites Dermanyssus gallinae collected from 32 batten- cage farms of laying hens localised in 14 voivodships. The following insecticides were used: Alfasekt (α-cypermetrin), Blaxime (phoxim), ByeMite (phoxim), Ficam (bendiokarb), Galtox B (fenitrotion, dichlorphos, α-cypermetrin), Knox-out (diazinon - microcapsule form), Master (chlorpyrifos - microcapsule...
The objective of the present research was the analysis of chosen factors contributing to mortality of ewes and their lambs from a flock naturally infected with maedi-visna virus (MVV). 1,406 mother sheep and their 2,234 progeny of two synthetic prolific- meat lines BCP and SCP and Suffolk breed were investigated. Summing up the research results concerning mortality of dams and lambs in a MVV-infected...
The cvtoprotective effect of silibinin in course of cytotoxicity induced by lasalocid had been measured in rat hepatoma FaO cell line. In the course of the study, MTT test (cellular metabolism), coomassie brillant blue binding test - CBB (total cellular proteins), and LDH release test (membrane integrity) were applied. In addition, changes in the cell morphology after 24 h treatment were observed...
In the study, phenotypic and genotypie properties of 42 Corynebacterium pseudotuberculosis strains isolated from 16 goat flocks were determined by random amplified polymorphic DNA and amplified fragment length polymorphism techniques. Several sets of RAPD primers and AFLP protocols were experimentally evaluated for potential of generating amplification profiles of the best discriminatory power. The...
Two trials were conducted. The aim of the first trial, performed under field conditions, was the comparison of the rearing performance between the chickens injected in ovo with 1.9 mg of raffinose family oligosaccharides (RFOs) (experimental group E), and chickens administered with the antibiotics (control group C). Environmental and feed conditions were similar for both groups. The mortality, feed...
This paper is focused on cloning and bioinformatical as well as immunological characterisation of the new vaccine antigen candidate against fasciolosis - Fasciola hepatica phosphoglycerate kinase (FhPGK). The antigen was cloned from the adult fluke by the use of RACE-PCR method. It was found that FhPGK is not a secretory and not a stage specific protein. It is present in all kinds of parasite tissue,...
The purpose of the study was to define if anaesthetic action of xylazine could conceivably result from the potentiation of inhibitory neurotransmitters or the inhibition of excitatory neurotransmitter systems in the brain. Rats were injected with xylazine at a dose of 50 mg/kg b.w., and then the hippocampus and thalamencephal were removed at 0.1, 0.25, 0.5, 1, 1.5, 2, 4, and 6 h after the injection...
The 61,020 samples of swine serum and vaginal swabs were collected in 2007-2009. The sera were examined by complement fixation test (CFT). Positive results were found in 277 pigs. The percentage of positive serological results in subsequent years was: 0.4%, 0.47%, and 1.65%, respectively. The vaginal swabs (n=277) selected from pigs diagnosed as positive in the CFT were tested by PCR. Occurrence of...
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